EFFECT OF INTERLEUKINS 2, 7, 15 ON THE PROLIFERATION OF NATURAL KILLERS IN VITRO

Introduction. The actual task of modern adoptive cancer immunotherapy is the selection optimal composition cytokines for ex vivo stimulation immunocompetent cells subsequent administration to oncological patients. purpose study was compare effect interleukin (IL) 2, 7, 15 and their combinations on proliferation natural killer in breast (BC) patients vitro. Materials methods. research conducted isolated by magnetic separation from mononuclear peripheral blood ten with locally advanced BC (stage II). After separation, were cultured at a concentration 2.5 × 105 / ml 10 days RPMI 1640 medium supplemented 40 ng each five experimental variants: IL?2; IL?7; IL?15; IL?7 IL?2 IL?15. On 10th day cultivation, phenotype cell cycle studied flow cytometry. For immunophenotyping cells, we used monoclonal antibodies antigens: CD3, CD16 56, CD45, CD4, CD19, CD8. stained propidium iodide. Results . final cultivation number living expressed as percentage seeding numbers significantly different control (45.9 %) samples (86.8 IL?15 (85.6 %), (76.4 (75.8 %). proportion killers (CD16+CD56+) differed (18.2 (45.6 (39.9 (36.2 (35.9 propor? tion T (CD3+ CD16+CD56+) (0.4 incu? bated (2.06 (2.2 (0.9 (1.26 (2.46 All tests also showed significant increase S?phase index (G2 M + S). Conclusion. maximum vitro obtained alone ?c?cytokines.